How to take DNA swabs

How to take DNA swabs

Center for Companion Animal Health, School of Veterinary Medicine

University of California-Davis

One Shields Avenue

Davis, California 95616-8782

Phone (530) 752-7295, Fax (530) 752-770

INSTRUCTIONS FOR COLLECTING BUCCAL CELLS FOR FIP RESEARCH

1. Complete the FIP questionnaire form to list your sample submissions for the three study groups.

2. Sample one cat at a time. If samples are to be collected from several cats in the same session, complete steps 1 – 7 for one cat before sampling the next.

3. Label EACH envelope containing swabs with the following: The date the swabs were taken The cat’s name The cat’s registration number if available or other identification Which study group the samples are for: Group 1 – Cats affected by FIP Group 2 – Relatives to cats affected by FIP Group 3 – Cats from bloodlines with absolutely no history of FIP.

4. Use double ended cotton swabs or cytological brushes for each cat. Place swab head against the inside of the cheek and gums, swirl vigorously 8 – 10 times. Use both ends of the cotton swabs.

5. Wave cotton swab or brush in the air or set aside in dry place until thoroughly dried.

6. Try to collect 3 – 4 swabs per cat.

7. Place swabs into the labeled paper envelope and seal. Do not wrap swabs in plastic wrap or seal in plastic bag.

8. Place all samples, submission form, identification information (pedigrees) and any other related reports or test results into one larger envelope and send to Niels Pedersen at U. C. Davis.

(NOTE: if you have blood, tissue or feces samples to forward, please contact Dr. Pedersen regarding the best method for sending those samples.)

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